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Blood Sciences

Blood Test Information

ENA Typing
(a.k.a Blotting, Enhanced Autoantibody Screen, Immunoblotting)
minimum sample volume required ~ 5ml

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Special instructions
Time limit for add on = 2 days

Interfering substances = None known

Repeat testing not normally recommended

Reference range

Linked to
ENA Screen

Turnaround Time
14 days

Department: Immunology

Clinical Application
ENA typing is performed by immunoblotting. Three blotting profiles are available. The appropriate choice of profile is dependant on clinical information, if provided, and initial screening results.

The myositis immunoblotting profile screens for: Anti-OJ, EJ, PL-12, PL-7, SRP, Jo-1, Pm-Scl75, Pm-Scl100, Ku, SAE, NXP-2, MDA5, TIF-1g, Mi-2 and Ro-52.

The ANA immunoblotting profile screens for: Anti-AMA-M2, Ribosomal-P, Histone, Nucleosome, ds-DNA, PCNA, CENP-B, Jo-1, Pm-Scl, Scl-70, Ro-60, Ro-52, La, U1-RNP and Sm.

The systemic sclerosis (nucleolar) immunoblotting profile screens for: Anti-Scl-70, CENP A, CENP B, RNAP III, U3-RNP, NOR90, Th/To, Pm-Scl100, Pm-Scl75, Ku, PDGFR and Ro-52.


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